ABSTRACT
Objective: To study the chemical constituents of metabolites of Alternaria sp. IS275, an endophytic fungus isolated from Ligusticum chuanxiong. Methods: The extraction fraction of ethyl acetate from endophytic fungi fermentation broth of L. chuanxiong were isolated and purified by silica gel, Sephadex LH-20, reversed phase medium pressure chromatography, preparative TLC, and semi-preparative RP-HPLC. The structure of the separated chemical constituent was elucidated by modern spectroscopy technology. Results: A new lactone compound (+)-4,11-dihydroxy-2-dodecene-5-lactone was isolated from the ethyl acetate fraction of the metabolite of Alternaria sp. IS275, an endophytic fungus of L. chuanxiong. Conclusion: Compound 1 is a new lactone compound, named ligualterlide A.